Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing.

ArciTect™ crRNA is a custom CRISPR RNA (crRNA), one of two synthetic RNA components required to make a guide RNA (gRNA) template for CRISPR-Cas9 genome editing. ArciTect™ crRNA contains a user-specified 19 to 21 base sequence complementary to the genomic location to be cleaved, and a linker sequence complementary to ArciTect™ tracrRNA (Catalog #STMC76016), allowing fast and efficient gRNA duplex formation. ArciTect™ crRNA should be designed so that it is directly upstream of a protospacer adjacent motif (PAM) site (NGG). It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency.