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Mouse IL-6 ELISA Kit
  ANTIA302538-96
 :  ANTIBODIES.COM
New Product
 :  
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Mouse IL-6 ELISA Kit
  ANTIA302538-96
 :  ANTIBODIES.COM
New Product
 :  

 

  • Pk:
    96 Tests
  • Assay duration:
    Multiple steps
  • Assay Type:
    Sandwich
  • Format:
    Pre-coated
  • Host:
  • Primary antibody reactivity:
    Mouse
  • Target protein:
    IL-6
  • Description:
    Mouse IL-6 ELISA kit
  • Environmentally Preferable:
  • Sample type:
    Serum, plasma, tissue homogenates, and other biological fluids
  • Cross reactivity:
    Mouse IL-6 ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse IL-6. No significant cross-reactivity or interference between IL-6 and analogues was observed.
  • Detection method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Shelf life:
    Store for 6 months at 4 °C
  • Detection range:
    6,25 - 400 pg/ml
  • Storage temperature:
    4 °C
  • Sample volume:
    100 μl
  • Sensitivity:
    3,75 pg/ml
  • Regulatory status:
    RUO

 

 

Mouse IL-6 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-6 in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-to-use ELISA kit
  • Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Mouse IL-6 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse IL-6 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for IL-6 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-6 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-6 Antibody, which binds the captured IL-6 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-6 captured in each well. The concentration of IL-6 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.