Serum-free medium and supplement for the measurement of drug toxicity on erythroid cells.

Screen large numbers of novel drug compounds for their effect on the proliferation and erythroid-specific differentiation of human hematopoietic stem and progenitor cells (HSPCs). Compared to using the colony-forming unit (CFU) assay, the liquid culture-based HemaTox™ assay is more flexible; add test compounds at various points throughout the culture and measure their effects on more primitive or more mature progenitor cells. The HemaTox™ Erythroid Kit promotes the proliferation of CD34+ HSPCs and their differentiation into cells expressing erythroid markers such as CD71 and CD235a.

Users can choose the readout method to quantify the response and estimate the IC50 and IC90 of each compound tested. Test up to 160 conditions - in triplicate - per kit in 5×96-well plates. This kit may be used on its own or in combination with HemaTox™ Myeloid Kit or HemaTox™ Megakaryocyte Kit to assess lineage-specific drug toxicity in parallel.