Serum-free media for differentiation of human PSCs to cardiomyocytes and long-term maintenance of human PSC-derived cardiomyocytes.

STEMdiff™ ventricular cardiomyocyte differentiation kit includes a medium for differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells (human pluripotent stem cells [hPSCs]) into ventricular cardiomyocytes (cardiac troponin T-positive [cTnT+]), as well as a medium for maintenance of hPSC-derived cardiomyocytes. This serum-free kit can be used to generate ventricular cardiomyocytes derived from a clump culture of hPSCs maintained in mTeSR™1, mTeSR™ plus, TeSR™-AOF, or TeSR™-E8™. Greater than 80% of these cells will be cTnT+. An average of 1×10⁶ cells can be harvested from a single well of a 12-well plate. STEMdiff™ cardiomyocyte maintenance kit comprises the maintenance basal medium and supplement; it can be used for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.

NOTE: This product was formerly named STEMdiff™ cardiomyocyte differentiation kit; the product itself and manufacturing procedures have not changed, but the name has been updated to more accurately reflect the cell type generated.