Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAml3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAml3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAml3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.

Flow Cytometry: 0,5 - 1 µg/10^6 cells IF: 1 - 2 µg/ml IHC (FFPE): 0,5 - 1 µg/ml (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAml3 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10 mM Tris with 1mM EDTA, pH9, for 10 - 20 min followed by cooling at RT for 20 min.

Type: Primary
Antigen: MAML3
Clonality: Monoclonal
Clone: MMLP3-1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human