- Antibody type:Primary
- Antigen name:ATF2 Thr51 + Thr53
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 750
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human
- Western blot:Yes
- Environmentally Preferable:
- Form:Liquid
- Gene ID:11909
- Antigen synonyms:cAMP response element binding protein CRE BP1|cAMP Response Element Binding Protein 2|ATF2 phospho Thr51 + Thr53|cAMP-dependent transcription factor ATF-2|CREBP1|HB 16|Activating Transcription Factor 2|p-ATF2 phospho T51 + T53|ATF2 protein|ATF 2|CREB2|Atf-2|cAMP-responsive element-binding protein 2|ATF2_HUMAN|Cyclic AMP-responsive|CRE-BP|CREB 2|Cyclic AMP dependent transcription factor ATF 2|CRE BP1|p-ATF2 phospho Thr51 + Thr53|ATF2 phospho T51 + T53
- Modification:Phosphorylation (Thr51+Thr53)
- Storage buffer:Aqueous buffered solution containing 0,01 M TBS (pH 7,4) with 1% BSA, 0,03% Proclin300 and 50% Glycerol
- Molecular weight:54 kDa
- Storage temperature:Store at −20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping temperature:4 °C
- Immunogen:KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Thr51+Thr53
- Tested applications:ICC
- Purification:Purified by Protein A
- Pack type:Vial
- Pk:100 µl
ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
Type: Primary
Antigen: ATF2 Thr51 + Thr53
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 750
Public Immunogen Range:
Host: Rabbit
Isotype: IgG
Reactivity: Human