- Antibody type:Primary
- Antigen name:Calpain 2
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 680
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Mouse
- Western blot:Yes
- Environmentally Preferable:
- Epitope:301-400/700
- Form:Liquid
- Gene ID:824
- Antigen synonyms:CALP80|CANP L2|Millimolar calpain.|CANP2|Calpain-2 large subunit|Calpain 2 catalytic subunit|Calpain large polypeptide L2|CANP 2|Calpain 2 large catalytic subunit|Calpain 2 m/II large subunit|CAPN2|Capa2|CANPL2|Calpain 2 large subunit|CAPN 2|M type|Calcium-activated neutral proteinase 2|M calpin|Calpain M-type|Calpain2|mCANP|FLJ39928|Millimolar-calpain|Calcium activated neutral proteinase 2| Calpain 2, large [catalytic] subunit|Calpain M type|M-calpain|Calpain-2 catalytic subunit|M calpain|Calcium activated neutral proteinase|CAN2_MOUSE|CANPL 2|CANPml
- Modification:Unmodified
- Storage buffer:Aqueous buffered solution containing 0,01 M TBS (pH 7,4) with 1% BSA, 0,03% Proclin300 and 50% Glycerol
- Molecular weight:78 kDa
- Storage temperature:Store at –20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping temperature:4 °C
- Immunogen:KLH conjugated synthetic peptide derived from mouse Calpain 2
- Tested applications:ICC
- Purification:Purified by Protein A
- Pack type:Vial
- Pk:100 µl
Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Type: Primary
Antigen: Calpain 2
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 680
Public Immunogen Range: 301-400/700
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse