- Antibody type:Primary
- Antigen name:SRC
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 750
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western blot:Yes
- Environmentally Preferable:
- Epitope:2-100/541
- Form:Liquid
- Gene ID:6714
- Antigen synonyms:c SRC|ASV|Oncogene SRC|protooncogene SRC Rous sarcoma|Neuronal SRC|Src|Neuronal CSRC tyrosine specic protein kinase|pp60c src|p60 Src|Tyrosine protein kinase SRC 1|SRC Oncogene|p60-Src|SRC_HUMAN.|Tyrosine kinase pp60c src|pp60csrc|V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian|Proto-oncogene c-Src|SRC_MOUSE|p60Src|CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA|Protooncogene SRC|OTTHUMP00000174476|Avian sarcoma virus|vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog|v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian|pp60c-src|SRC1|Proto oncogene tyrosine protein kinase Src|OTTHUMP00000174477|Proto-oncogene tyrosine-protein kinase Src|Tyrosine protein kinase SRC1|v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog|EC 2.7.10.2|OTTHUMP00000030931|cSrc
- Modification:Unmodified
- Storage buffer:Aqueous buffered solution containing 0,01 M TBS (pH 7,4) with 1% BSA, 0,03% Proclin300 and 50% Glycerol
- Molecular weight:61 kDa
- Storage temperature:Store at –20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping temperature:4 °C
- Immunogen:KLH conjugated synthetic peptide derived from human SRC
- Tested applications:ICC
- Purification:Purified by Protein A
- Pack type:Vial
- Pk:100 µl
c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labelling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
Type: Primary
Antigen: SRC
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 750
Public Immunogen Range: 2-100/541
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat