RPMI 1640 Medium, with Glutamine, without Sodium Bicarbonate, MP Biomedicals

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers.

Used with a serum supplement
Shown to support several cell lines in the absence of serum
Used in fusion protocols and in the growth of hybrid cells

As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.

Environmentally Preferable:

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