SIGMAFAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity.

• Each tablet dissolved in 10 ml deionised water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9,5

Common uses are in immunoblotting or dot blotting. It is less commonly used in immunohistochemistry. These tablets require no additional buffers or steps to prepare an active substrate solution.