- Pk:100 µG
- Protein/peptide type:Recombinant
- Source:HEK293 cells
- Species:Human
- Environmentally Preferable:
- Biological activity:Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha at 5 ug/mL ( 100 ul/well ) can bind Humira with a linear range of 0.04-0.6 ng/mL. The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D. The ED50 < 25 pg/mL, corresponding to a specific activity of >40000000 Unit/mg.
- Protein synonyms:DIF|TNFα|cachexin|cachectin|TNF-alpha|TNFSF2|TNFA
- Protein/peptide name:TNF alpha
- Purity:>95% as determined by SDS-PAGE.
- Molecular weight:17.4 kDa
- Formulation:Lyophilized, PBS, pH7.4
- Tested applications:Western Blot
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.