This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.