Pierce™ Magnetic RNA-Protein Pull-Down Kit is a streamlined, robust method to enrich protein-RNA interactions using end-labelled RNA as the bait.

• Captures ribonucleoprotein complexes directly with end-labelled RNA
• Does not use or require antibodies for pull-down
• No spin cups or centrifugation necessary for the enrichment of RBP
• Procedure streamlined for minimal hands-on time (less than 3 hours) after RNA labelling reaction
• Used in vitro transcribed RNA or synthetic RNA for labelling of various lengths and complexity
• Low bead background and unrelated RNA or mutated RNA does not significantly enrich specified RBPs

Magnetic RNA-Protein Pull-Down Kit provides reagents to efficiently enrich RNA Binding Proteins (RBPs) using RNA end-labelled with desthiobiotin and streptavidin magnetic beads. This direct enrichment of the protein-RNA interaction provides an alternative to antibody capture of protein-RNA complexes or moieties incorporated into the nucleic acid. The kit is amenable to several downstream applications, including Western blotting and mass spectrometry. It also finds applications in mutational analysis, structure function analaysis and identification of RNA-protein interactions and MS analysis of unknown RNA-protein binding pairs or complexes.