Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionised water or equivalent to its final working concentration. This buffer consists of 1,0 M Tris HCl, 1,5 M sodium chloride and 0,1% (v/v) Tween-20 at a pH of 7,5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionised water.

10X TTBS solution was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.