Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

• Immobilised anti-c-Myc monoclonal antibody binds the c-Myc epitope tag with high specificity, providing high yield immunoprecipitation products and clean Western blot detection
• Excellent results with as little as 1 µg of anti-c-Myc antibody in IP mode with the positive control lysate
• Kit includes a positive control lysate that contains over-expressed GST-c-Myc to validate the reagents and specific protocol, and the instructions provide tips for planning other controls
• Affinity system is compatible with IP or Co-IP from various cell lysates and physiological (non-denaturing) buffer systems
• Complete kit includes all necessary reagents, convenient spin columns, and easy-to-follow instructions

The high affinity anti-c-Myc antibody coupled agarose enables immunoprecipitation (IP) of c-Myc-tagged proteins or co-immunoprecipitation (Co-IP) of their interacting partners without antibody contamination. The Pierce™ spin columns provided with the kit handle small volumes of anti-c-Myc agarose and allow the entire procedure to be performed in a single spin column, preventing anti-cMyc agarose loss between washes. The spin columns further enhance recovery by efficiently separating buffer from the anti-c-Myc agarose.