The TnT® T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins using an extract derived from the Spodoptera frugiperda Sf21 cell line.

Protein synthesis reactions are initiated by the addition of a DNA template, eliminating the need for the time-consuming process of in vitro RNA synthesis. All components necessary for the transcription/translation are present in the TnT® T7 ICE master mix. To initiate protein synthesis, the only component that must be added is the DNA template. Reactions are complete within 4 hours.