About this item
G-Biosciences' Fab Fragmentation kits are designed for the generation and isolation of Fab fragments from IgG molecules
Papain is a cysteine protease enzyme that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis. Immobilized Papain offers the advantage of generating Fab and Fc fragments without the need to remove the papain enzyme after digestion.
Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. The Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected due to the spin-format design.
In addition, SpinOUT™ GT-600 desalting columns are supplied to ensure the initial antibody sample is in the optimal condition for Fab Fragmentation. The Fab Fragmentation kit is optimized for mouse, rabbit and human IgG, using 0.25-4 mg/ 0.5 mL sample. The Fab Fragmentation (Micro) kit is optimized for mouse, rabbit and human IgG, using 25-250 µg/ 125 µL sample.
The kits utilize our Immobilized Papain resin.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Environmentally Preferable: