About this item
No compromises. Unparalleled performance meets sustainability.
- Unrivaled sensitivity - detection down to two copies of RNA
- Sustainable and earth friendly: Lyophilized eQo bead format eliminates dry ice, CO₂ consumptions and reduces shipping costs
- Novel reverse transcriptase: Convenient, ambient reaction setup, overcomes rigid RNA secondary structure
- High stability: Up to one year at room temperature, up to one year rehydrated at −20 °C
- Tough Tested: Broad and significantly enhanced tolerance to PCR inhibitors
eQo 1-step ToughMix is a lyophilized reagent system for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes. It is supplied with a proprietary rehydration buffer that when combined with the lyophilized 'eQo beads' produces a stabilized 4X concentrated master mix.
The kit includes thermolabile UDG for amplicon carryover elimination, an enhanced warm-start reverse transcriptase (RT) and all other required components for 1-step RT-qPCR except RNA template and primer/probe(s). The reaction chemistry has been optimized for inhibitor tolerance and delivers exceptional performance in either single-plex or highly demanding multiplex 1 step RT-qPCR formats.
Cycler compatibility: BioRad CFX, Quantabio Q, Roche LightCycler 480
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Application:
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