The NEXTFLEX™ universal blockers block non-specific hybridization, enhancing specificity and reducing sequencing costs. They are compatible with all ligation and tagmentation workflows for Illumina®

• Effective in blocking single-index or dual-index libraries
• Compatible with all NEXTFLEX™ barcodes and all other ligation-based and tagmentation-based workflows for Illumina® sequencing platforms
• Simplifies and reduces the cost of target capture without sacrificing performance
• 96 barcode blockers available

During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule. The NEXTFLEX™ universal blockers are designed to prevent this concatemerization of library molecules. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases. The NEXTFLEX™ universal blockers are designed for use with indexed adapters for Illumina sequencing platforms. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI). The NEXTFLEX Universal Blockers are compatible with ligation-based workflows, such as NEXTFLEX™ or Illumina trusequence library prep kits. They are also compatible with tagmentation-based workflows, such as Illumina Nextera library prep kits.