The Monarch® RNA cleanup kit enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.

• RNA recovery of 70 to 100%, even with inputs <20 ng
• Efficiently purify RNA ≥25 nt (a simple modification enables purification of RNA ≥15 nt)
• Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
• Simplified workflow with a single wash buffer
• Unique column design prevents buffer carryover and elution of silica particulates
• Columns and buffers are available separately
• Purified RNA is ready for use in a wide variety of downstream applications

This kit rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (>25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl for concentrated RNA. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥15 nts).

Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT).

Monarch RNA Cleanup kits are also available for 50 µg (NEB #T2040) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.