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Specifications
- Application:
- Method / Format:Extraction for direct PCR
- Prep size:Mini
- Refine target molecule:Total RNA
- Sample type:Cultured cells
- Target molecule:RNA
- Description:QuickExtract™ RNA Extraction Kit
- Size:50 mL
- Environmentally Preferable:
- Description:QuickExtract™ RNA Extraction Kit
- Cat. No.:76081-774
Specifications
About this item
Rapid, simple RNA extraction (no purification) from mammalian cells, ready for RT-PCR applications
- Fast: Extract end-point and real-time RT-PCR ready RNA in minutes from low to high numbers of samples
- Compatible: Extracted RNA is compatible with both real-time and endpoint RT-PCR assays
- Automation-friendly: Simple extraction protocol makes incorporation into an automated workflow easy
- Flexible: When necessary, a DNase I digestion step can be added for sensitive downstream applications
- Safe: No toxic reagents used
The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis. The result is easy processing of one to hundreds of samples in minutes, with no sample loss or toxic organic solvents. The QuickExtract RNA Extraction Solution works with cultured adherent and suspension cells including buccal cells, and has been tested on human, mouse, rat, E. coli, and S. aureus cell cultures. It is not suitable for tissue samples or plant samples. An optional DNase I treatment may improve certain downstream applications.