Formats: 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µl reaction volume (5 µl of sample). 5000 assay point kit allows you to run 5000 wells in 96-well or 384-well format, using a 50 µl reaction volume (5 µl of sample). Features: no-wash steps, no separation steps, ELISA alternative technology, sensitive detection, broad sample compatibility, small sample volume, results in less than 3 hours, half the time of an ELISA assay. Plasminogen activator inhibitor-1 (PAI-1) is a single chain glycoprotein of 50 kDa. PAI-1 is secreted and is mainly produced by the endothelium, liver, and adipose tissue. It is also produced by certain tumours. PAI-1 activity is tightly regulated on the transcriptional level by transforming growth factor ß. PAI-1 is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA), which convert plasminogen to plasmin. Congenital PAI-1 deficiency can cause hemorrhagic diathesis. PAI-1 is present in larger amounts in various pathologies, such as in a number of cancers or obesity. In breast cancer, high levels of PAI-1 are associated with a high risk of recurrence. AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated donor beads while another anti-analyte antibody is conjugated to AlphaLISA acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the acceptor beads, resulting in a sharp peak of light emission at 615 nm.
- Harmonized Code US-3822005090
- H phrases US-H302-H315-H318-H317-H402-H412
- P phrases US-P261-P280a-P280i-P273-P264-P270-P272-P305+P351+P338-P310a-P301+P312a-P363-P332+P313-P333+P313-P337+P313-P330-P302+P352a-P321-P362+P364-P501a
- GHS-Signal words - US-Danger
- GHS-Symbols - US-GHS05 GHS07
Human Plasminogen Activator Inhibitor-1 (PAI-1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The analyte consists of the active glycosylated human PAI-1. This standard is already provided in the AlphaLISA human PAI-1 kit, but can be ordered separately.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Environmentally Preferable: