E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors.

• A Powerful Tool for Identifying and Characterizing Polypeptides
• Reduced chance of expressed proteins degrading
• Includes all components needed for coupled transcription/translation
• Produces very low levels of endogenous proteins, thus low backgrounds

The E. coli S30 Extract for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors, providing a powerful tool for identifying and characterizing polypeptides. The investigator needs only to supply the cloned DNA containing the appropriate prokaryotic promoter and ribosome binding sites. The S30 Extract for Circular DNA Templates is prepared from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity. This results in a greater stability of expressed proteins that would otherwise be degraded by proteases if expressed in vivo. The S30 in vitro system also allows highe expression levels of proteins that are normally expressed at low levels in vivo due to the action of host-encoded repressors.

This results in a greater stability of expressed proteins that would otherwise be degraded by proteases if expressed in vivo. The S30 in-vitro system also allows higher expression levels of proteins that are normally expressed at low levels in vivo due to the action of host-encoded repressors.

The S30 Extract for Circular DNA Templates is prepared by modifications of the method described by Zubay from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity.