Urea Agar Slant (Christensen’s Urea) is useful for the detection of urease production in bacteria.
- Detects urease production in bacteria
- Rapid growth
- Fast reaction time
The slant contains urea and phenol red as the pH indicator
Proteus species rapidly hydrolyze urea, and a positive reaction is usually seen within one to six hours. Other organisms perform this reaction slower and may require 24 to 48 hours or longer incubation time.
The pH indicator turns from pale yellow to pink-red when cabable organisms hydrolyze urea form ammonia and turn the medium alkaline. The reduced buffer content and peptone in this medium promote more rapid growth and reaction time for many members of the Enterobacteriaceae. Dextrose is included in the formulation to stimulate urease activity in organisms that hydrolyze urea slowly, and to exclude false-negative reactions.
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