To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:PDE5A/PDE5
- Description:Human PDE5A/PDE5 ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human PDE5A/PDE5 ELISA kit exhibits high specificity and excellent specificity for the detection of human PDE5A/PDE5. No significant cross-reactivity or interference between PDE5A/PDE5 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.156 - 10 ng/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.094 ng/ml
- Regulatory Status:RUO
- Cat. No.:76739-832
Specifications
About this item
Human PDE5A/PDE5 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human PDE5A/PDE5 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 0.156 - 10 ng/ml
- Sensitivity: 0.094 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human PDE5A/PDE5 ELISA kit (A77106) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human PDE5A/PDE5 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for PDE5A/PDE5 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PDE5A/PDE5 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PDE5A/PDE5 Antibody, which binds the captured PDE5A/PDE5 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PDE5A/PDE5 captured in each well. The concentration of PDE5A/PDE5 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.