To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Application:
- Method / Format:Magnetic beads
- Prep size:Scalable
- Refine target molecule:poly(A) RNA
- Sample type:Cultured cellsHuman/animal cells and tissues
- Target molecule:RNA
- Description:Magnetic mRNA Isolation Kit
- Size:25 isolations
- Environmentally Preferable:
- Description:Magnetic mRNA Isolation Kit
- Cat. No.:101444-828
- Supplier no.:S1550S
The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents.
The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. Oligo d(T)25 Magnetic Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound dT DNA as a primer in a first-strand cDNA reaction.