The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

• Fast - all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
• No expensive hardware or vacuum required; no clogging issues
• Optimised protocol makes Western blot analysis easier
• No expensive consumables or extra equipment required
• Kit stable for 1 year stored at 4 °C

This complete kit provides all the reagents for blocking, antibody dilution and to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands on time and yields results comparable to classic Western blotting with ECL. The included ECL substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.