Anti-UBB Rabbit Polyclonal Antibody

ROCK200-401-431

Anti-UBB Rabbit Polyclonal Antibody

ROCK200-401-431

Antibody type:
Primary
Antigen name:
Ubiquitin B
Antigen symbol:
UBB
Clonality:
Polyclonal
Conjugation:
Unconjugated
ELISA:
Yes
Host:
Rabbit
ImmunoPrecipitation:
Yes
Isotype:
Reactivity:
Multiple
Western blot:
Yes
Environmentally Preferable:
Cross adsorption:
none
Format:
Antibody fraction
Form:
Lyophilized
Gene ID:
7314
Storage buffer:
0,02 M Potassium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,01% (w/v) Sodium Azide,
Storage temperature:
Cool
Concentration:
5,0 mg/ml by UV absorbance at 280 nm
Shipping temperature:
Ice
Immunogen:
This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.
Purification:
multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
Size:
500 µg
Pk:
500 µG

This purified polyclonal antibody reacts with ubiquitin by ELISA and western blot. Although not tested, this antibody is likely functional in immunochemistry and immunoprecipitation. For detection of free Ub by western blotting use Tris-Tricine SDS-PAGE.

Recommended Dilutions: ELISA: 1:1000 - 1:5000; Western Blot: 1:200 - 1:1000;
Restore with deionized water (or equivalent); 100 µl

Type: Primary
Antigen: UBB
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Pan