Supplier: Cytiva

Resource RPC are prepacked SOURCE 15RPC columns designed for rapid screening experiments, method development, and small-scale protein purification using reversed phase chromatography.

Supplier: EPPENDORF

Engineered to minimise sample binding and elevate recovery, Eppendorf® Protein LoBind® tubes safeguard valuable proteins during processing. The cutting-edge LoBind® technology ensures optimal yields for Western blotting, mass spectrometry, and protein purification applications.

Supplier: Cytiva

HiTrap Heparin HP columns are designed for high-resolution purification of DNA-binding proteins, coagulation factors, and other plasma proteins.

Supplier: AAT BIOQUEST

Anti-human secondary antibodies have well-characterised specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target.

Supplier: Cytiva

HiScreen Capto SP ImpRes column is packed with strong cation exchange modern resin for fast high resolution protein purification in process development.

Supplier: Cytiva

Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.

Supplier: MERCK PRODUCTION CHEMICALS

Imidazole is a critical buffer substance for bioprocesses to enhance the purity of your target protein. Its most widely used application is the preparation of buffer solutions to operate downstream purification columns, packed with resins.

Supplier: Cytiva

The HiScreen MabSelect SuRe column uses protein A chromatography for antibody purification. This protein A column is prepacked with MabSelect SuRe affinity resin and is part of our process development platform. These packed columns are used for method optimization and parameter screening for capture of monoclonal antibodies.

Supplier: Corning

Axygen® Axyspin is a high-performance refrigerated microcentrifuge designed to quickly process samples for applications such as nucleic acid and protein preparation, purifications, extractions and pelleting.

Supplier: Cytiva

Capto™ Phenyl (high sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification.

Supplier: Cytiva

GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimised for use in Western blotting with ECL™ Detection Reagents. The conjugate recognises multiple epitopes of GST and therefore is not reliant on functional GST for fusion protein detection. It has also been cross-absorbed to E. coli proteins so that GST-fusion proteins can be easily and specifically identified against an E. coli background.

Supplier: Cytiva

HiTrap Capto Phenyl ImpRes is prepacked with Capto Phenyl ImpRes media for screening and small-scale protein purifications using hydrophobic interaction chromatography.

Supplier: Thermo Fisher Scientific

Thermo Scientific EZ-Link NHS-Desthiobiotin is a short, membrane permeable, amine-reactive labeling reagent whose biotin-like group is elutable from streptavidin, making it ideal for intracellular protein labeling and purification.

Supplier: Cytiva

HiTrap IMAC FF is prepacked with IMAC Sepharose 6 Fast Flow. The resin is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Supplier: Cytiva

HiScreen™ IMAC FF columns for optimising purification of histidine-tagged proteins by Immobilised Metal ion Affinity Chromatography (IMAC).

Supplier: Thermo Fisher Scientific

The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.

Supplier: Cytiva

HiTrap Capto DEAE is a weak anion exchanger prepacked with BioProcess Capto resins for screening and small-scale protein purifications using ion exchange chromatography (IEX)..

Supplier: Merck Millipore (Novagen)

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Supplier: Cytiva

HiTrap Butyl HP are prepacked Butyl Sepharose High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.

Supplier: Thermo Fisher Scientific

Columns are designed for the separation and purification of hydrophilic anionic proteins and peptides.

Supplier: Cytiva

HiTrap Capto Q chromatography column is prepacked with a strong anion exchange modern resin for small scale protein purification.

Supplier: Cytiva

Capto™ Phenyl (High sub) and Capto Butyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification.

Supplier: G-Biosciences

The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

Supplier: Thermo Fisher Scientific

Pierce™ cellulose acetate filter spin cups are convenient tools for manipulating small volumes of affinity supports (20 - 400 µl) for protein purification. Simply add the affinity resin and sample to one of the spin cups, then use a microcentrifuge to efficiently wash away contaminants and elute the purified sample without losing any resin in the process. Spin cups allow more protein to be affinity purified in less time.

Supplier: ProSci Inc.

Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.

Supplier: Cytiva

The Untagged column package includes cation/anion exchange and size exclusion chromatography columns for purification of untagged/native proteins. The columns provided in the package are prepacked with Capto Q ImpRes and Capto SP ImpRes high-performance resins and can be used individually or in combination.

Supplier: Thermo Fisher Scientific

Pierce glutathione coated plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein. As the plates are pre-blocked, initial purification of the cell lysates is not necessary.

Supplier: Cytiva

These columns are prepacked with MabSelect™ PrismA protein A chromatographyn resin that has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing efficient cleaning between purification runs. These columns are suitable for purifying antibodies with affinity chromatography in research and process development.

Supplier: Cytiva

HiTrap™ SP FF is pre-packed with SP Sepharose™ Fast Flow, and is a strong cation exchanger for small scale protein purifications as well as screening of binding and elutions conditions.

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.