Kjeldahl nitrogen determination
Nitrogen determination is a critical process often used to calculate protein levels in various food products. The Kjeldahl method is one of the most reliable methods for nitrogen determination and involves three key stages:
- Digestion. A food sample is treated with concentrated sulfuric acid to break down organic material and release nitrogen. The nitrogen is then converted into ammonium sulfate. This step is crucial for breaking down complex proteins into simpler forms, making the nitrogen more accessible for measurement.
- Distillation. After digestion, the sample undergoes distillation. During this process, nitrogen is separated from the remaining sample in the form of ammonia and collected in a receiving solution to be prepared for quantification.
- Titration. Finally, the collected ammonia is titrated with a standard acid solution. This step allows precise measurement of the nitrogen content, which can be used to calculate the protein level in the food sample.
The Kjeldahl method's reliability and precision make it a standard procedure in food quality control. This technique helps ensure that consumers are provided accurate information about the protein content of their food.
Product selection
We offer different heating systems varying in sample and volume (from 100–800 ml) capacity.
Avantor® offers distillation systems to meet every budget.
Everything from simple burettes to fully automated, programmable titration systems from SCHOTT and Mettler Toledo.
Catalysts are used to increase the boiling temperature and reduce reaction times. Choose from leading suppliers of tables and reagents, including tablets from the VWR® Chemicals portfolio.
Need more information about Kjeldahl equipment and supplies?
Contact us today to discuss your needs. Our team is ready to help.