Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:Tau
- Description:Human Tau ELISA kit
- Environmentally Preferable:
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human Tau ELISA kit exhibits high specificity and excellent specificity for the detection of human Tau. No significant cross-reactivity or interference between Tau and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:7,813 - 500 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:4,688 pg/ml
- Regulatory status:RUO
Specifications
About this item
Human Tau ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Tau in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 7,813 to 500 pg/ml
- Sensitivity: 4,688 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra assay: CV <8%, Inter assay: CV <10%
Human Tau ELISA kit (A78417) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Tau in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Tau has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Tau present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Tau Antibody, which binds the captured Tau present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Tau captured in each well. The concentration of Tau can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.