These magnetic agarose beads consist of highly crosslinked agarose beads embedded with magnetite and a covalently attached tetradentate nitrilotriacetic acid (NTA) chelator charged with divalent nickel ions. They provide a fast, convenient method for purification of His-tagged recombinant proteins.

• High capacity: Sufficient for both routine and demanding purification procedures
• Binds >70 mg of 6xHis-tagged protein per ml of settled resin
• High-performance and reproducible beads: Non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
• Low non specific binding: Optimised purification protocol results in better tagged-protein purification
• Versatile: Purify proteins using native or denaturing conditions
• Compatible: Use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives

The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added feature of magnetic handling. Magnetic agarose beads are a valuable tool for small-scale (~1 mg) purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications with agarose chromatography supports.

The beads are incubated with cell lysate containing His-tagged protein and then magnetically separated from the supernatant manually or through automation using an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Nonspecifically bound protein can be washed away before dissociating bound His-tagged protein with elution buffer. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.