Human recombinant pro-BDNF (from E. coli)
PRSI91-063
:
- Pk:50 µG
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Environmentally Preferable:
- Storage conditions:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Protein synonyms:Brain-Derived Neurotrophic Factor|BDNF|Abrineurin
- Protein/peptide name:pro-BDNF
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:52 kD
- Sequence:Ala19-Arg247
- Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 250mM NaCl, pH 7.2. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
- This recombinant protein can be used for biological assays. For research use only.