Pierce™ Polyhis-Protein Interaction Pull Down Kit
PIER21277
:
- Pk:1 KIT
- Description:His protein interaction pull-down kit, sufficient for at least 25 pull-down reactions, each using 25 µl of resin
- Environmentally Preferable:
The Pierce™ his protein interaction pull-down kit contains the necessary components to capture and purify proteins that interact with his-tagged fusion proteins.
- Complete kits provide all components and detailed protocols for purifying protein:protein interactions
- No special equipment needed use common laboratory equipment and reagents
- Microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
- Instructions include protocols for use with bait and prey proteins expressed from a variety of sample types
- Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding
- Binds 10 to 25 mg of histidine-tagged fusion protein per ml of resin
Applications include discover a new protein:protein interaction from a cell lysate; confirm a putative interaction from a cell lysate or with a previously purified protein; extract protein:protein interaction information from in vitro transcription/translation lysates.
: Complete kit includes cobalt chelate agarose resin, 750 µl; pull-down lysis buffer, 250 ml; imidazole solution (4 M), 5 ml; TBS pack (makes 500 ml); spin columns and accessories, 27 columns; and microcentrifuge collection tubes, 100 tubes.