PS, NeutrAvidin™ coated plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

• Low nonspecific binding
• Easy and gentle immobilisation of biotin-containing conjugates
• No denaturing of the protein component of a conjugate upon binding
• Pre-blocked with Blocker™ BSA or SuperBlock™ blocking buffer and ready-to-use
• High binding capacity plates provides wider detection range and better curve linearity for small biotinylated ligands

Clear plates for colorimetric ELISA and other assays. White plates for chemiluminescent. Black plates for fluorescent assays.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Thermo Scientific NeutrAvidin protein is avidin that has been deglycosylated to prevent nonspecific lectin-binding. With a near-neutral pI (6,3), this results in lower nonspecific interactions in certain applications compared with streptavidin. NeutrAvidin protein lacks the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells.

Thermo Scientific NeutrAvidin Protein Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Features of NeutrAvidin protein coated plates include white plates for chemiluminescent detection methods pre-blocked with blocker BSA buffer or SuperBlock blocking buffer easy and gentle immobilisation of biotin-containing conjugates low nonspecific binding no denaturing of the protein component of a conjugate upon binding ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene less expensive than streptavidin coated plates lowest nonspecific binding properties of all biotin-binding proteins for research use only. Not for use in diagnostic procedures.