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Agarose and Acrylamide

Agarose and acrylamide are the most commonly used electrophoresis gels for their versatility with buffers and ability to generate reproducible results. Agarose typically runs horizontal tests to resolve large DNA fragments while acrylamide runs vertical separations for shorter nucleic acids. Easy to handle, the gels are chosen based on the high resolution desired and the samples. The concentration or ratio of the agarose and acrylamide will determine the overall working porosity.

VWR® Acrylamide and Bis-Acrylamide

VWR® Acrylamide and Bis-Acrylamide

Supplier: VWR Chemicals

An extensive line of acrylamide and bis-acrylamide pre-weighed powder blends, pre-mixed stock solutions and ready to use solutions for customised polyacrylamide gel electrophoresis of proteins and PAGE of nucleic acids.

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VWR® Acrylamide-​Bis Solution (37,5:1) 30% Aqueous Solution, Ultra Pure Grade

VWR® Acrylamide-​Bis Solution (37,5:1) 30% Aqueous Solution, Ultra Pure Grade

Supplier: VWR Chemicals

Electrophoresis Gels, Acryl/Bis™, Acrylamide-​Bis solution (37,5:1) 30% aqueous solution

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VWR® Acryl/Bis™ Acrylamine/Bis-Acrylamine

VWR® Acryl/Bis™ Acrylamine/Bis-Acrylamine

Supplier: VWR Chemicals

A 40% (w/v) solution of ultra pure acrylamide (38,96%) and bis-acrylamide (1,04%) at a final ratio of 37.5:1.

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Acryl/​Bis solution (30%) 29:1, ultrapure

Acryl/​Bis solution (30%) 29:1, ultrapure

Supplier: VWR Chemicals

Electrophoresis Gels, Acryl/Bis™, Acryl/​Bis solution (30%) 29:1

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VWR®, Agarose I™

VWR®, Agarose I™

Supplier: VWR Chemicals

Agarose I™ is a standard melting/gelling agarose, suitable for routine nucleic acid analytical/preparative applications (250 bp to 22 kb).

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VWR®, Agarose RA™

VWR®, Agarose RA™

Supplier: VWR Chemicals

Agarose RA™ is a general application agarose that provides good resolution and is cost effective for high volume users. It was developed specifically for quick checks of PCR products, plasmids preps, screening and cloning.

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Peqlab peqGOLD, Agarose, Universal

Peqlab peqGOLD, Agarose, Universal

Supplier: VWR Chemicals

High quality standard agarose for routine analysis with broad separation range (0,05 to 50 kbp). Ideal for blotting applications due to high gel strength. Free from RNases, DNases and enzyme inhibitors.

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GelGreen® Agarose LE

Supplier: Biotium

Ultrapure molecular biology grade agarose pre-coated with GelGreen® Nucleic acid gel stain for convenient and safer preparation of precast gels.

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Protein A Agarose (Endotoxin-free)

Protein A Agarose (Endotoxin-free)

Supplier: Abcam

Reusable, Endotoxin free Protein A conjugated Agarose beads with ≥15 mg/ml binding capacity.

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Agarose LE, ultra-pure molecular bology grade

Supplier: Biotium

High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with GelRed®, GelGreen®, and DNAzure® gel stains.

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Agarose I, Pierce™

Agarose I, Pierce™

Supplier: Thermo Fisher Scientific

Agarose I (molecular biology grade) is a highly purified and carefully blended formulation that provides uniform lot-to-lot consistency for preparation of electrophoresis gels.

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Agarose, SeaKem® LE

Agarose, SeaKem® LE

Supplier: LONZA

SeaKem® LE is a high gel strength, low EEO, standard gelling temperature agarose perfect for electrophoresis and other molecular biology applications.

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Melt & Pour UltraSpec-Agarose™

Supplier: EDVOTEK

0.8% UltraSpec-Agarose™ prepared with TAE buffer. Simply melt, cool and pour.

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Agarose, MetaPhor™

Agarose, MetaPhor™

Supplier: LONZA

Intermediate melting point agarose ideal for resolution of DNA fragments, PCR and RT-PCR products, differing in size by 2%, in the range of 20 to 800 bp, by submarine gel electrophoresis.

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Agarose, SeaPrep™

Supplier: LONZA

Ultra-soft agarose, ideal for high efficiency hybridoma cloning and expansion of cDNA libraries.

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Agarose, I.D.NA™

Agarose, I.D.NA™

Supplier: LONZA

I.D.NA™ Agarose is a high gel strength, low electroendosmosis agarose developed for consistent resolution of DNA in DNA identity testing.

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Agarose II, low melting point, Pierce™

Supplier: Thermo Fisher Scientific

Agarose II (low melt) is a highly purified and carefully blended formulation that provides uniform lot-to-lot consistency for preparation of electrophoresis gels that require a lower melting temperature.

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Agarose, NuSieve® 3:1

Agarose, NuSieve® 3:1

Supplier: LONZA

NuSieve® 3:1 is a high strength agarose ideal for analytical electrophoresis of small DNA/RNA fragments and in-gel PCR/transformation/ligation.

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UltraSpec-Agarose™, DNA electrophoresis grade

UltraSpec-Agarose™, DNA electrophoresis grade

Supplier: EDVOTEK

Electrophoresis Gels, UltraSpec-Agarose™

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Agarose, SeaKem® HGT

Agarose, SeaKem® HGT

Supplier: LONZA

SeaKem® HGT agarose is a high-gelling temperature (42 °C) agarose that exhibits high gel strength and clarity. It is primarily used in protein electrophoresis applications such as counterimmunoelectrophoresis (CIEP) and crossed immunoelectrophoresis.

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Agarose, SeaKem® HEEO

Agarose, SeaKem® HEEO

Supplier: LONZA

SeaKem® HEEO Agarose is a very high EEO agarose that is useful in applications requiring significant cathodal migration, such as IEP of IgG and IgM. It can also be used to blend with a lower EEO agarose to achieve a specific EEO value.

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Agarose, SeaKem® Gold

Agarose, SeaKem® Gold

Supplier: LONZA

SeaKem® is a high gel strength, low EEO, standard gelling temperature agarose perfect for electrophoresis and other molecular biology applications. This Genetic Technology Grade™ (GTG) Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE).

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Agarose, NuSieve® Genetic Technology Grade™ (GTG)

Agarose, NuSieve® Genetic Technology Grade™ (GTG)

Supplier: LONZA

NuSieve® Genetic Technology Grade™ (GTG) Agarose is a low melting temperature (65 °C) agarose that finely resolves DNA fragments, PCR and RT-PCR products ranging from 10 to 1000 bp.

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Agarose, IsoGel™

Agarose, IsoGel™

Supplier: LONZA

IsoGel™ Agarose is a highly purified agarose specifically manufactured for isoelectric focusing (IEF). It has no measurable EEO due to a manufacturing process which minimises fixed anions and mobile cations. IsoGel™ Agarose IEF Plates are sufficiently rigid to be cast in vertical tubes in addition to vertically moulded and horizontally open cast thin gels. Gels cast on GelBond® film dry down quickly and easily to transparent permanent records.

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Agarose, SeaKem® Genetic Technology Grade™ (GTG)

Agarose, SeaKem® Genetic Technology Grade™ (GTG)

Supplier: LONZA

SeaKem® is a high gel strength, low EEO, standard gelling temperature agarose perfect for electrophoresis and other molecular biology applications.

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Agarose, SeaKem® HE

Agarose, SeaKem® HE

Supplier: LONZA

SeaKem® HE agarose is a high EEO agarose that provides enhanced resolution in immunoelectrophoresis (IEP), crossed-IEP, counterimmunoelectrophoresis (CIEP), and serum protein electrophoresis.

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Acrylamide/Bis-Acrylamide

Supplier: Thermo Fisher Scientific

Premix of acrylamide with the common cross-linking reagent of final ratio 29:1.

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Acrylamide/ Bis-Acrylamide

Supplier: G-Biosciences

Acrylamide (electrophoresis grade) is supplied as a powder or a 40% solution in ultrapure water. Bisacrylamide (Bis (N,N’-methylenebisacrylamide)) (electrophoresis grade) is supplied as a powder or a 2% solution in ultrapure water.

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Agarose, low gelling temperature

Supplier: Thermo Fisher Scientific

Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.

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Agarose, Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

This agarose is a granular, free-flowing powder.

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