3540 Results for: "Sulfo-gels"
Sulfo-GMBS
Supplier: Bachem Americas
Sequence: Sulfo-N-succinimidyl 4-maleimidobutyrate sodium salt
Synonym(s): Sulfo-GMBS sodium salt
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Sulfo-SMA Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
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Sulfo-DIBMA Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
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Sulfo-NONOate disodium salt ≥90%
Supplier: Enzo Life Sciences
Negative control for NO releasing NONOates.
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[[(3,5-Dimethyl-4-sulfo-1H-pyrrol-2-yl)(3,5-dimethyl-4-sulfo-2H-pyrrol-2-ylidene)methyl]methane](difluoroborane)disodium salt ≥96.0% (by HPLC)
Supplier: TCI America
CAS Number: 121461-69-6
Molecular Formula: C14H17BF2N2O6S2
Molecular Weight: 466.19
Purity/Analysis Method: >96.0% (HPLC)
Form: Crystal
Lambda max.: 491 nm (H2O)
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Sulfo-SMCC (3-Sulfo-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt)
Supplier: TCI America
CAS Number: 92921-24-9
MDL Number: MFCD00054978
Molecular Formula: C16H18N2O9S
Molecular Weight: 436.37
Form: Crystal
Color: White
Storage Temperature: <0°C
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Ponceau S
Supplier: VWR
A substitute for acid fuschin in Van Gieson stain. Also used for staining proteins after electrophoresis.
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Mal-sac-HNSA
Supplier: Bachem Americas
Sequence: ε-Maleimidocaproic acid-(2-nitro-4-sulfo)-phenyl ester · sodium salt
Synonym(s): mal-sac-HNSA
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5-sulfo-4'-diethylamino-2,2'-dihydroxyazobenzene
Supplier: TCI America
[Reagent for Aluminum]
CAS Number: 1563-01-5
MDL Number: MFCD00024960
Molecular Formula: C16H19N3O5S
Molecular Weight: 365.40
Form: Crystal
Color: Red
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Tropaeolin O 0.04% (w/v) in aqueous solution indicator
Supplier: Ricca Chemical
Tropaeolin O 0.04% (w/v) in aqueous solution indicator
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Ponceau S 0.1 % (w/v) in 5% acetic acid staining solution
Supplier: Biotium
Ponceau S staining solution is used for the detection of proteins on PVDF and nitrocellulose membranes with a limit of detection of about 250 ng of transferred protein.
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Ponceau S ≥85.0% (by total nitrogen basis) for biochemical research
Supplier: TCI America
[for Biochemical Research]
CAS Number: 6226-79-5
MDL Number: MFCD00003892
Molecular Formula: C22H16N4O13S4
Molecular Weight: 760.55
Purity/Analysis Method: >85.0% (N)
Form: Crystal