203 Résultats pour : « Promocell »

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Primary Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request). They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin. New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.

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PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells

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Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.

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Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process.

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Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs. They are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation.

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The PromoCell Melanocyte Growth Medium is a serum-free, PMA (Phorbol Myristate Acetate) containing medium optimized for the cultivation of human melanocytes

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Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor. (Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.) The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.

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PromoCell Human CD14+ Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14+ Monocytes are isolated by positive selection with > 95% purity.

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The PromoCell Chondrocyte Growth Medium is designed for the optimal cultivation of human chondrocytes

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The PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum (4% V/V) medium

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Primary Human Uterine Microvascular Endothelial Cells (HUtMEC) are isolated from the myometrium (middle layer of the uterine wall) from a single donor who is not pre-treated with hormones. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.

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Primary Human Coronary Artery Endothelial Cells (HCAEC) are isolated from the coronary arteries (the right and the left coronary artery, including the anterior descending and the circumflex branches) from a single donor

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Primary Human Cardiac Microvascular Endothelial Cells (HCMEC) are isolated from heart ventricles from a single donor. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and Willebrand factor and negative for smooth muscle alpha-actin.

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The Monocyte Attachment Medium allows for the efficient adherence selection of Monocytes from freshly isolated Mononuclear Cells (MNC) while maintaining optimal cell health

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This DetachKit 2 is used for very gentle, safe and efficient detachment of primary human cells in routine subculturing.

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Primary Human Renal Cortical Epithelial Cells (HRCEpC) are isolated from the cortex of the human kidney and stain positive for cytokeratin.

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Primary Human Uterine Fibroblasts (HUF) are isolated from the uterine wall

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Primary Human Uterine Smooth Muscle Cells (HUtSMC) are isolated from the myometrium (the middle layer of the uterine wall) and stain positive for smooth muscle α-actin

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PromoCell hMSC-UC are harvested from normal human umbilical cord matrix (Whartons jelly). The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts.

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Primary Human Aortic Endothelial Cells (HAoEC) are isolated from the human ascending (thoracic) and descending (abdominal) aorta

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Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs.

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PromoCell Small Airway Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the distal respiratory system

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Trypsin is the most widely employed enzyme used for cell detachment

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Primary Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from the arteries of the umbilical cord and are often used for in vitro studies on atherosclerosis but also for endothelial cell-dependent lymphocyte activation and chemoattractant activity experiments

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Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of an adult heart

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The PromoCell Dendritic Cell (DC) Generation Medium has been developed for the easy and efficient generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes

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Primary Human Coronary Artery Smooth Muscle Cells (HCASMC) are isolated from human coronary arteries and stain positive for smooth muscle α-actin. Coronary arteries supply the heart with blood and obstruction in atherosclerosis causes myocardial infarction.

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The PromoCell Macrophage Generation Medium was developed for easy and efficient generation of M1- or M2-polarized macrophages from freshly isolated peripheral blood monocytes

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Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of juvenile foreskin and adult skin (different locations)

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Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas.