279 Results for: "CUBE BIOTECH"

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1E3D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: CUBE BIOTECH

Each membrane protein has its native lipid environment. Therefore, each lipid environment is unique to each membrane protein. Moreover, it is this lipid environment that synthetic polymers like AASTYs interact with. Therefore, one needs a broader selection of polymers since there is not one AASTY that fits each phospholipid composition. To address this question, AASTYs come in three different ratios of acrylic acid to styrene. The ratios differ in hydrophilic property of AASTY, which results in different affinities to varying membrane lipid compositions. The second feature to screen for when it comes to AASTY is the size of the resulting nanodiscs. One of AASTY's most excellent features, compared to SMA or DIBMA, is that its nanodiscs are pretty uniform in size. Therefore, the correct fitting nanodisc size must be determined before.

Supplier: CUBE BIOTECH

PureCube epoxy activated MagBeads are your best option to couple biomolecules that carry free-standing amine (NH₂) and thiol (SH) groups.

Supplier: CUBE BIOTECH

The HisCube MagBead protein purification MINI kit is designed to meet the demands of your laboratory for efficient and high-quality small-scale His-tag protein purifications.

Supplier: CUBE BIOTECH

PureCube amine carboxy agarose MagBeads are your best option to couple biomolecules that carry free-standing Amine (NH₂) groups.

Supplier: CUBE BIOTECH

The FPLC Cartridge assembly press is an efficient tool that simplifies the packaging process for FPLC columns.

Supplier: CUBE BIOTECH

AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-45 - are highly alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-45 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: CUBE BIOTECH

AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-50 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made using controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: CUBE BIOTECH

AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-50 show higher thermodynamic stability.

Every membrane protein solubilization needs to undergo a screening process in before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all.

We recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 dh5 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

Membrane scaffold protein, human origin.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

PureCube Ni-IDA MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 dh5 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

Mild, non-ionic detergents such as Tridecylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Supplier: CUBE BIOTECH

Mild, non-ionic detergents such as Decylglucoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.

Supplier: CUBE BIOTECH

Our E. coli cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Supplier: CUBE BIOTECH

Our E. coli cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Supplier: CUBE BIOTECH

PureCube Alprenolol resin provides highly-specific purification β-adrenergic receptors with maximum protein yield.

Supplier: CUBE BIOTECH

PureCube Maleimide MagBeads are your best option to couple biomolecules that carry thiol (SH) groups.

Supplier: CUBE BIOTECH

PureCube Fe-NTA MagBeads were developed to enrich phosphorylated proteins. They can also be used to purify His-tagged proteins as a secondary function.

Supplier: CUBE BIOTECH

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Supplier: CUBE BIOTECH

PureCube NTA XL (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.

Supplier: CUBE BIOTECH

PureCube amine activated agarose resin is your best option to couple biomolecules carrying free-standing carboxy (COOH) groups.

Supplier: CUBE BIOTECH

PureCube epoxy agarose XL resin is your best option to couple biomolecules that carry thiol (SH) groups.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

Membrane scaffold protein; rat origin.

Supplier: CUBE BIOTECH

The PureCube NTA (nitrilotriacetic acid) Compact Cartridge was developed for IMAC-based protein purification and protein enrichment methods.

Supplier: CUBE BIOTECH

Enhanced GFP (eGFP) is a modified version of GFP that exhibits even stronger fluorescence for easier detection.