279 Results for: "CUBE BIOTECH"
SMALP BZ30 Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
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T. californica Native AChR
Supplier: CUBE BIOTECH
Our AChR is directly purified from the acetylcholine receptor of Torpedo californica.
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Nanodisc Assembly Kit MSP1D1-His POPC
Supplier: CUBE BIOTECH
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
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Human Recombinant MSP1E3D1 (from E. coli)
Supplier: CUBE BIOTECH
Membrane scaffold protein, human origin.
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PureCube StrepTactin® HiCap StrepTactin® Agarose
Supplier: CUBE BIOTECH
PureCube StrepTactin® agarose resin beads consist of 6% cross-linked agarose coupled to the streptactin protein.
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Human Recombinant MSP2N2-D73C (from E. coli)
Supplier: CUBE BIOTECH
Membrane scaffold protein, human origin.
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N-Octyl-β-D-glucopyranoside ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Octylglucopyranoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.
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DIBMA 10 in HEPES, PureCube for protein solubilisation
Supplier: CUBE BIOTECH
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
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Ultrasolute™ Amphipol 17
Supplier: CUBE BIOTECH
The Ultrasolute family evolved from the first amphipol A8-35 is used for highly efficient solubilization and stabilization of membrane proteins without the use of detergents while preserving their activity. They demonstrate tolerance to moderate concentrations of divalent cations and exhibit no absorption at wavelengths >250 nm.
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PureCube Co-NTA MagBeads
Supplier: CUBE BIOTECH
PureCube Co-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.
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PureCube Compact Cartridge Glutathione
Supplier: CUBE BIOTECH
The protein purification with the PureCube Glutathione agarose and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.
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PureCube Compact Cartridge Zn-NTA
Supplier: CUBE BIOTECH
The PureCube Zn-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Zn-NTA resin. You can choose between two sizes, 1 and 5 ml bed volume.
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SARS-CoV-2 Recombinant SARS CoV-2 Spike Protein in DIBMA Glycerol (from
Hek293 Expi cells)
Supplier: CUBE BIOTECH
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
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Halobacterium salinarum S10 Recombinant Sensory Rhodopsin-1 (from E. coli)
Supplier: CUBE BIOTECH
Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria.
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SARS-CoV-2 Recombinant SARS-CoV-2 Spike Protein in SMALP 140 (from HEK293 cells)
Supplier: CUBE BIOTECH
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
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PureCube Co-NTA Agarose
Supplier: CUBE BIOTECH
Our PureCube Co-NTA agarose resins are tiny agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells.
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PureCube Ti-NTA MagBeads
Supplier: CUBE BIOTECH
PureCube Ti-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.
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PureCube Amine Activated MagBeads XL
Supplier: CUBE BIOTECH
PureCube Amine Activated MagBeads XL are your best option to couple biomolecules carrying free-standing carboxy (COOH) groups.
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PureCube Glutathione MagBeads XL
Supplier: CUBE BIOTECH
The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.
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Sulfo-SMA Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
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N-Tridecylphosphocholine ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.
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PureCube Butyl Agarose
Supplier: CUBE BIOTECH
Our PureCube butyl agarose resin is a suspension of purification beads for hydrophobic interaction chromatography.
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Nanodisc MSP1D1 dH5 DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
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Nanodisc Msp1D1-His DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
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Nanodisc MSP1D1 DMPC
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
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HighSpec Rho Starter Set 1: HighSpec Rho1D4 Agarose + Rho1D4 Peptide
Supplier: CUBE BIOTECH
HighSpec Rho1D4 agarose resin is a suspension of 40 µm agarose beads, coupled to an anti-Rho1D4 antibody. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen.
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SMALP BZ40 Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Expand 3 Items
Nanodisc Assembly Kit MSP1E3D1-His POPC
Supplier: CUBE BIOTECH
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1E3D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
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SMALP BZ35 Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
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AASTY Screening Kit
Supplier: CUBE BIOTECH
Each membrane protein has its native lipid environment. Therefore, each lipid environment is unique to each membrane protein. Moreover, it is this lipid environment that synthetic polymers like AASTYs interact with. Therefore, one needs a broader selection of polymers since there is not one AASTY that fits each phospholipid composition. To address this question, AASTYs come in three different ratios of acrylic acid to styrene. The ratios differ in hydrophilic property of AASTY, which results in different affinities to varying membrane lipid compositions. The second feature to screen for when it comes to AASTY is the size of the resulting nanodiscs. One of AASTY's most excellent features, compared to SMA or DIBMA, is that its nanodiscs are pretty uniform in size. Therefore, the correct fitting nanodisc size must be determined before.