279 Results for: "CUBE BIOTECH"

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

Our PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

Supplier: CUBE BIOTECH

HighSpec Rho1D4 agarose XL resin is a suspension of 400 µm agarose beads coupled to an anti-Rho1D4 antibody.

Supplier: CUBE BIOTECH

The HisCube His-tag protein purification MINI Kit is our way to supply your laboratory with a convenient method for high-quality, small-scale His-tag protein purifications. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow.

Supplier: CUBE BIOTECH

The HisCube His-tag protein purification MIDI Kit is our way to supply your laboratory with a convenient method for high-quality, small-scale His-tag protein purifications. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow.

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

Channelrhodopsin 1 is a 7-transmembrane protein that acts as a light-driven cation channel in eukaryotic green algae.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: CUBE BIOTECH

Our AChR is directly purified from the acetylcholine receptor of Torpedo californica.

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

Membrane scaffold protein, human origin.

Supplier: CUBE BIOTECH

PureCube StrepTactin® agarose resin beads consist of 6% cross-linked agarose coupled to the streptactin protein.

Supplier: CUBE BIOTECH

Membrane scaffold protein, human origin.

Supplier: CUBE BIOTECH

Octylglucopyranoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.

Supplier: CUBE BIOTECH

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Supplier: CUBE BIOTECH

The Ultrasolute family evolved from the first amphipol A8-35 is used for highly efficient solubilization and stabilization of membrane proteins without the use of detergents while preserving their activity. They demonstrate tolerance to moderate concentrations of divalent cations and exhibit no absorption at wavelengths >250 nm.

Supplier: CUBE BIOTECH

PureCube Co-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: CUBE BIOTECH

The protein purification with the PureCube Glutathione agarose and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

Supplier: CUBE BIOTECH

The PureCube Zn-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Zn-NTA resin. You can choose between two sizes, 1 and 5 ml bed volume.

Supplier: CUBE BIOTECH

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Supplier: CUBE BIOTECH

Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria.

Supplier: CUBE BIOTECH

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Supplier: CUBE BIOTECH

Our PureCube Co-NTA agarose resins are tiny agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells.

Supplier: CUBE BIOTECH

PureCube Ti-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.

Supplier: CUBE BIOTECH

PureCube Amine Activated MagBeads XL are your best option to couple biomolecules carrying free-standing carboxy (COOH) groups.

Supplier: CUBE BIOTECH

The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.