279 Results for: "CUBE BIOTECH"
Nanodisc Rat MSP1D1-His DMPG
Supplier: CUBE BIOTECH
Membrane scaffold protein. Rat origin.
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Nanodisc MSP1E3D1 DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
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Nanodisc Rat MSP1E3D1-His DMPG
Supplier: CUBE BIOTECH
Membrane scaffold protein. Rat origin.
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PureCube Compact Cartridge Ni-NTA
Supplier: CUBE BIOTECH
The PureCube Ni-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Ni-NTA resin.
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PureCube Glutathione MagBeads
Supplier: CUBE BIOTECH
The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.
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PureCube Ni-NTA MagBeads
Supplier: CUBE BIOTECH
PureCube Ni-NTA MagBeads were developed by Cube Biotech for His-tag protein purification.
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Anti-Strep Antibody
Supplier: CUBE BIOTECH
This is an antibody specifically for targeting a Strep-(WSHPQFEK)-tag on a protein.
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Anti-Strep Antibody (HRP (Horseradish Peroxidase))
Supplier: CUBE BIOTECH
This is an antibody specifically for targeting a Strep-(WSHPQFEK)-tag on a protein.
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PureCube Compact Cartridge Cu-NTA
Supplier: CUBE BIOTECH
The PureCube Cu-NTA compact cartridge is an IMAC chromatography column prepacked with PureCube Cu-NTA resin. You can choose between two sizes, 1 ml bed volume and 5 ml bed volume.
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Halobacterium salinarum S9 Native Bacteriorhodopsin
Supplier: CUBE BIOTECH
Bacteriorhodopsin (BR) is a 7-transmembrane protein that acts as a light-driven proton pump in archaebacteria.
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N-Undecylphosphocholine ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.
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N-Nonylphosphocholine ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.
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PureCube 100 NTA Agarose
Supplier: CUBE BIOTECH
PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 100 µm.
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Nanodisc MSP1E3D1-His POPC
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
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Nanodisc MSP1E3D1-His DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
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PureCube Epoxy Activated Agarose
Supplier: CUBE BIOTECH
PureCube Epoxy Agarose resin is your best option to couple biomolecules that carry free-standing amine or thiol groups.
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Anti-GST Antibody
Supplier: CUBE BIOTECH
This is an antibody specifically for targeting a GST-tag on a protein.
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PureCube Compact Cartridge Co-NTA
Supplier: CUBE BIOTECH
The PureCube Co-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Co-NTA resin.
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Synthetic Rho1D4 Peptide
Supplier: CUBE BIOTECH
Elution of Rho1D4-tagged protein from matching purification beads.
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Glyco-DIBMA in HEPES, PureCube for protein solubilisation
Supplier: CUBE BIOTECH
Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.
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SMALP BZ Screening Kit
Supplier: CUBE BIOTECH
SMALP BZs are synthesized via RAFT polymerization, achieving a better-defined polymer size and a low dispersity.
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DIBMA glucosamine in HEPES, PureCube for protein solubilisation
Supplier: CUBE BIOTECH
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
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N-Tetradecylphosphocholine ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.
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Octyl-β-D-thioglucopyranoside ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Octylthioglucoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10.
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AASTY 6-55
Supplier: CUBE BIOTECH
AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-55 - are highly-alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-55 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general, lighter AASTYs, like 6-55 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.
The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.
Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022
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Nanodisc Assembly Kit MSP1D1 dH5 POPC
Supplier: CUBE BIOTECH
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 dh5 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
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Nanodisc Assembly Kit MSP1E3D1-His DMPC
Supplier: CUBE BIOTECH
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1E3D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
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Nanodisc Assembly Kit MSP1D1 DMPC
Supplier: CUBE BIOTECH
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
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Nanodisc MSP2N2 DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
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n-Decyl-β-D-maltoside ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Decylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.