279 Results for: "CUBE BIOTECH"

Supplier: CUBE BIOTECH

PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

Supplier: CUBE BIOTECH

PureCube Zn-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: CUBE BIOTECH

Our PureCube octyl agarose resin is a suspension of purification beads for Hydrophobic Interaction Chromatography (HIC).

Supplier: CUBE BIOTECH

PureCube NHS (N-hydroxy succinimide) agarose XL resin is your best option to couple biomolecules carrying free-standing amine groups.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP2N2 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

HighSpec Rho1D4 MagBeads XL is a suspension of 90 µm magnetic agarose beads coupled to an anti-Rho1D4 antibody.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

Membrane scaffold protein, mouse origin.

Supplier: CUBE BIOTECH

Our Rho1D4 starter set 3 combines our HighSpec Rho1D4 compact cartridge and the Rho1D4 elution peptide.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

PureCube 100 Ni-INDIGO agarose resin is a unique development by Cube Biotech for His-tag protein purifications of the highest quality.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

IPTG is a modified sugar that can be used to induce protein expression under the control of the lacUV promotor. Our IPTG is of highest quality and purity. Like allolactose, IPTG binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner, thereby allowing the transcription of genes in the lac operon. But unlike allolactose, the sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from metabolizing or degrading the inducer. The concentration of IPTG therefore remains constant and the expression of lac p/o-controlled genes would not be inhibited during the experiment.

Supplier: CUBE BIOTECH

Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

HighSpec Rho1D4 MagBeads are a suspension of 30 µm magnetic agarose beads coupled to an anti-Rho1D4 antibody.

Supplier: CUBE BIOTECH

The Rho1D4 starters set 2 combines our HighSpec Rho1D4 MagBeads and the Rho1D4 elution peptide.

Supplier: CUBE BIOTECH

PolyHunter agarose was developed for the depletion of synthetic polymers used in solubilization steps.

Supplier: CUBE BIOTECH

DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Supplier: CUBE BIOTECH

HiCap StrepTactin® Compact Cartridge is a FPLC-ready column that is pre-packed with our PureCube HiCap StrepTactin® agarose resin.

Supplier: CUBE BIOTECH

Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Supplier: CUBE BIOTECH

PureCube Epoxy Agarose resin is your best option to couple biomolecules that carry free-standing amine or thiol groups.

Supplier: CUBE BIOTECH

This is an antibody specifically for targeting a GST-tag on a protein.

Supplier: CUBE BIOTECH

The PureCube Co-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Co-NTA resin.

Supplier: CUBE BIOTECH

Elution of Rho1D4-tagged protein from matching purification beads.

Supplier: CUBE BIOTECH

Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.

Supplier: CUBE BIOTECH

SMALP BZs are synthesized via RAFT polymerization, achieving a better-defined polymer size and a low dispersity.

Supplier: CUBE BIOTECH

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.