9449 Results for: "Biotium"

Supplier: Biotium

GelRed™ and GelGreen™ are a new generation of fluorescent nucleic acid gel stains designed to replace the highly toxic ethidium bromide (EtBr)

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

Supplier: Biotium

This antibody recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®640R is a far-red fluorescent dye (Ex/Em 642/662 nm) with excellent brightness, and the best photostabiity among spectrally-similar dyes.

Supplier: Biotium

This antibody recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®488A is a green fluorescent dye (Ex/Em 490/515 nm) with excellent brightness and photostability. The dye is minimally charged for less non-specific binding. CF®488A also is compatible with super-resolution imaging by TIRF.

Supplier: Biotium

This antibody recognizes a calcium-dependent complex of CD41/CD61, a dimer of 90 kDa and 140 kDa present on the membrane of normal platelets and megakaryocytes. CD41/CD61 is also known as platelet glycoprotein GPIIb/GPIIIa or integrin IIa/3. This complex is the receptor of fibrinogen, fibronectin and von Willebrand factor, and mediates platelet adhesion and aggregation.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®647 is a far-red fluorescent dye (Ex/Em 650/665 nm) with excellent brightness. It also is compatible with super-resolution imaging by STORM.

Supplier: Biotium

CD45RA Monoclonal antibody, Clone: PTPRC/1131, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF647, Immunogen: Recom full-length human CD45RA protein, Synonyms: B220, CD45R, Application: IF, IHC, FC, Size: 500 uL

Supplier: Biotium

This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

GnRH-Receptor (LHRH Receptor), Monoclonal antibody, Clone: GNRHR/768, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF640R, Immunogen: A synthetic peptide aa 1-29 from the N-terminal of human GnRH receptor, Application: IF, Flow, Size: 100uL

Supplier: Biotium

HLA-DRB, Monoclonal antibody, Clone: HLA-DRB/1067, Host: Mouse, Species reactivity: Human, Monkey, Isotype: IgG's, Conjugate: CF568, Immunogen: Activated human peripheral blood mononuclear cells (LN-3 & HLA-DRB/1067), Application: IF, IHC (formalin), Western, Flow, Size: 100uL

Supplier: Biotium

This antibody recognizes human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

Complement C4d Monoclonal antibody, Clone: C4D204, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF594, Immunogen: Recombinant human Complement 4d protein, Application: IF, Immunohistology (formalin), Size: 100uL

Supplier: Biotium

Cyclin B1 Monoclonal antibody, Clone: CCNB1/1098, Host: Mouse, Species reactivity: Mouse, Human, Isotype: IgG1, kappa, Conjugate: CF568, Immunogen: Recom human full-length CCNB1 protein, Synonyms: CCNB, CCNB1, Application: IF, IHC, FC, Size: 100uL

Supplier: Biotium

This antibody recognizes a protein of 55 kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkins disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®594 is a deep red fluorescent dye (Ex/Em 593/614 nm). It yields the brightest conjugates among spectrally similar dyes, and has excellent photostability.

Supplier: Biotium

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

VEGFR1 / Flt-1, Monoclonal antibody, Clone: FLT1/658, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF594, Immunogen: Recombinant human VEGF-R1 protein, Application: Immunofluorescence, Flow cytometry, Size: 500uL

Supplier: Biotium

In Western blotting, this antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®647 is a far-red fluorescent dye (Ex/Em 650/665 nm) with excellent brightness. It also is compatible with super-resolution imaging by STORM.

Supplier: Biotium

Von Willebrand Factor, Monoclonal antibody, Clone: 3E2D10, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF488A, Immunogen: Recombinant human vWF fragment spanning aa 845-949, Synonyms: Coagulation Factor VIII, Application: IF, Size: 500uL

Supplier: Biotium

Lambda Light Chain, Monoclonal antibody, Clone: HP6054, Host: Mouse, Species reactivity: Human, Isotype: IgG2a, kappa, Conjugate: CF488A, Immunogen: Purified IgG myeloma proteins coupled to polyaminostyren, Synonyms: BJP, Application: IF, Size: 100uL

Supplier: Biotium

Anti-IgG Chicken Polyclonal Antibody (CF™ 568)

Supplier: Biotium

MyoD1, Monoclonal antibody, Clone: 5.8A, Host: Mouse, Species reactivity: Mouse, Rat, Human, Chicken, Isotype: IgG1, kappa, Conjugate: purified, Immunogen: Recombinant mouse MyoD1 protein, Synonyms: bHLHc1, Application: IF, IHC (formalin), Flow, Size: 500uL

Supplier: Biotium

CD36 Monoclonal antibody, Clone: 185-1G2, Host: Mouse, Species reactivity: Human, Isotype: IgG2a, kappa, Conjugate: CF568, Immunogen: Stimulated human leukocytesUnigene 120949, Application: Immunofluorescence, Flow cytometry, Size: 100uL

Supplier: Biotium

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

This antibody recognizes a protein of 110 kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

Involucrin, Monoclonal antibody, Clone: IVRN/827, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: biotin, Immunogen: Purified involucrin from human keratinocytes, Synonyms: IVL, Application: Immunofluorescence, Flow cytometry, Size: 500uL

Supplier: Biotium

This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®647 is a far-red fluorescent dye (Ex/Em 650/665 nm) with excellent brightness. It also is compatible with super-resolution imaging by STORM.

Supplier: Biotium

IgA Immunoglobulin, Monoclonal antibody, Clone: IA761, Host: Mouse, Species reactivity: Human, Isotype: IgG1, Conjugate: CF543, Immunogen: Purified human alpha heavy chain, Synonyms: A2m Marker; Ig alpha 1 Chain C Region, Application: IF, IHC (formalin), Size: 100uL

Supplier: Biotium

Anti-IgG Goat Polyclonal Antibody (CF™ 514)

Supplier: Biotium

Ep-CAM / CD326 Monoclonal antibody, Clone: VU-1D9, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF568, Immunogen: Small cell lung carcinoma cells, Application: IF, Immunohistology (formalin), Western, FC, Size: 500 uL

Supplier: Biotium

This antibody recognizes a glycoprotein of 110 kDa, identified as CD26 (Workshop VI; Code: N-L039). It is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production. In Western blotting, this MAb reacts with only glycosylated CD26, but not with the deglycosylated form. It does not prevent ADA binding to CD26.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

ZAP70, Monoclonal antibody, Clone: ZAP70/528 + 2F3.2, Host: Mouse, Species reactivity: Human, Isotype: IgG's, Conjugate: CF568, Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70, Synonyms: Selective T cell defect, Size: 100uL