The Q5® High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format.

• For use in high-specificity PCR, high-fidelity PCR, cloning, long or difficult amplification and high-throughput PCR.

The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM MgCl2. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.