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Q5* High-Fidelity DNA Polymerase 100units
Q5® High-Fidelity DNA Polymerase, New England BioLabs
# de catalogue CA102500-138
Numéro CAS:  
Q5* High-Fidelity DNA Polymerase 100units
Q5® High-Fidelity DNA Polymerase, New England BioLabs
# de catalogue CA102500-138
Numéro CAS:  
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Spécifications

  • Concentration:
    2,000 units/ml
  • Description:
    Q5® High-Fidelity DNA polymerase
  • Size:
    100 Units
  • Cat. no.:
    CA102500-138

Spécifications

A propos de cet article

The Q5® High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format.

  • For use in high-specificity PCR, high-fidelity PCR, cloning, long or difficult amplification and high-throughput PCR.

The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM MgCl2. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.

Ordering information: The Q5 High-Fidelity DNA Polymerase and the Q5 Hot Start High-Fidelity DNA Polymerase are supplied with Q5 High GC Enhancer and Q5 Reaction Buffer.
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