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Spécifications
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:Typical limit of detection (LOD) for BDNF is less than 2 pg/ml, determined as 150% of the blank value.
- Target protein:BDNF
- Description:Mature BDNF Rapid ELISA Kit: Human, Mouse, Rat (1 Plate)
- Size:96 Tests
- Cross reactivity:No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/ml in assay buffer. The reactivity of full-length proBDNF (0.125 ng/ml – 5 ng/ml) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% ± 0.5% in weight (w/v) concentration, or 12.1% ± 1.2% in molar concentration (mean ± SEM)
- Specificity:Human, mouse, rat and numerous other species
- Detection range:7.8 - 500 pg/ml
- Cat. no.:75838-838
- Supplier No.:BEK-2211-1P
Spécifications
A propos de cet article
The Mature BDNF RapidTM ELISA Kit is a sandwich ELISA that allows the quantification of mature BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), pig serum, cell lysates, brain extracts, human milk and sheep CSF only if used as directed, with a simplified protocol and no loss of sensitivity or specificity.
Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples, human milk and CSF. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately. This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1% and 5%, respectively.This ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin.
The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF present in samples and protein standards. This Mature BDNF ELISA kit employs a recombinant human mature BDNF standard approved by the World Health Organization.
The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen.This ELISA kit has not been tested for other applications.
Fournisseur: VWR International
$81.84 to $877.92