To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Assay Type:Competitive
- Developmental time:90 - 120 min
- Mid point:~8 - 15 ng/ml (50% B/B0)
- Sensitivity:~3 ng/ml (80% B/B0)
- Detection range:1.17 - 150 ng/ml
- Storage temperature:–20 °C
- Incubation time:18 hr
- Cat. no.:75817-294
- Supplier No.:589324-96
- No. of Tests:96 Wells
Specifications
About this item
A competitive assay that can be used for the quantification of 5-methyl-2'-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices.
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure 5-methyl-2'-deoxy cytidine levels down to three ng/ml
- Read: Colorimetric at 405 to 420 nm
DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2'-deoxy-cytidine yielding the modified base 5-methyl-2'-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division.
Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2'-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual. For example, patients with leukemia excrete significantly elevated levels of 5-methyl-2'-deoxy cytidine compared to healthy individuals.
Global methylation within tissues can be measured in a similar manner, allowing study of tissue-specific changes that occur as a result of differentiation, aging, or carcinogenesis. Cayman’s DNA Methylation ELISA Kit is a competitive assay that can be used for the quantification of 5-methyl-2'-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices.