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Spécifications
- Description:FastSCRIPT™ cDNA Synthesis Kit
- Cat. no.:10002-278
- No. of Reactions:25
- Supplier No.:31-5300-0025R
Spécifications
A propos de cet article
The FastSCRIPT cDNA synthesis kit contains everything to generate unbiased, efficient, sensitive high-quality cDNA for downstream experiments
The kit contains a proprietary M-MLV reverse transcriptase (RT) molecule blended with an optimized RNase inhibitor, a mix that is both thermostable and extremely active.
This RTase mix synthesizes cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. The relative concentrations of random hexamers and anchored oligo(dT) primers have been designed specifically to generate cDNA optimized for use in real-time PCR experiments.
The FastSCRIPT cDNA synthesis kit can be used with total or poly(A)+ RNA, miRNA and viral RNA targets and provides sensitive detection of low copy number transcripts.
and removes the need for user optimization, due to its robust performance and ease of use.