IBI's Potato Dextrose Agar is recommended for the cultivation and isolation of yeasts and molds from dairy food products

Potato Dextrose Agar is composed of 4.0gm Potato Infusion, 20.0gm Dextrose, and 15.0gm Agar with a pH (@25°C) of 5.6 ± 0.2. To prepare, mix 39.0gm of the medium in 1L of purified water until evenly dispersed. Heat with repeated stirring and boil for 1 minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. If desired, the pH of the medium can be adjusted to 3.5 ± 0.1, prior to pouring plates, by the addition of 14ml of sterile 10% tartaric acid to melted sterile medium cooled to 45-50°C. Avoid reheating medium after the addition of the tartaric acid solution.

Store at room temperature, keep tightly sealed, and protect from moisture and light.

Lowering the pH to 3.5 ± 0.1 inhibits bacterial growth and aids in the isolation of fungi.